Evaluation of the gonococcal complement-fixation test.
نویسنده
چکیده
The gonococcal complement-fixation test (GCFT), based on the same principle as the Wassermann reaction (WR), has been in use for over 60 years. The test was applied for the first time, using a culture of the gonococcus as antigen, by Muller and Oppenheim (1906) to establish the diagnosis in a case of arthritis. Teague and Torrey (1907) suggested that negative results in the GCFT were due to different strains of gonococci and showed that a 'polyvalent' antigen consisting of several strains gave better results. Schwartz and McNeil (1911) confirmed the superiority of a 'polyvalent' antigen. They found that cases which gave negative results with an antigen made from six strains gave definite positive results when examined with an antigen made from twelve strains. Schwartz and McNeil (1912) considered that the GCFT was absolutely specific for the gonococcus because in it there was a true antigen-antibody combination. Kolmer and Brown (1914) thought that the reaction was not generally as satisfactory as the WR because the quantity of gonococcal antibody was much smaller than that of reagin unless the disease was widespread. Shupe (1916) believed that, while the test was not infallible, it was the most reliable method available to determine the absence of gonococci. He stressed that the results should be correlated with the other findings in the case. Weiss and Arnold (1938) showed that increasing the amounts of serum tested gave more frequent positive reactions in gonorrhoea without increasing non-specific reactions to a disturbing degree. The lower level of antigonococcal antibody in serum from a patient suffering from gonorrhoea as compared with anti-syphilitic antibody in the serum of a patient suffering from syphilis was probably due to the fact that the former infection was localized while the latter was generalized. An objection to using large amounts of serum in
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عنوان ژورنال:
- The British journal of venereal diseases
دوره 47 4 شماره
صفحات -
تاریخ انتشار 1971